Estudiantes de grado

Sofía Bertinetti, Estudiante Biotecnología
Agustin Lagger, Estudiante Biotecnología
Nazarena Villarreal, Estudiante Biotecnología

Autofagia y propiedades emergentes del metabolismo celular: un enfoque sistémico.

Nos centramos en los procesos y propiedades emergentes que definen los entornos celulares -particularmente el metabolismo carbono/nitrógeno y el estado redox- y su relación bidireccional con la autofagia. Consideramos la autofagia como parte integral del sistema de endomembranas interconectado y exploramos cómo estos entornos modulan la actividad autofágica y, recíprocamente, cómo su alteración impacta sobre dichas propiedades. Estudiamos tanto macroautofagia como microautofagia en Arabidopsis y Glycine max, con especial énfasis en su papel en interacciones simbióticas con rizobios.

Publicaciones

• “Differential effects of reactive oxygen species on autophagy in Arabidopsis”. Alejandro Enet, Germán Robert *, Laura Saavedra, Ramiro Lascano *. bioRxiv 2023; doi.org/10.1101/2023.08.11.552961
• «Autophagy modulates growth and development in the moss Physcomitrium patens» Georgina Pettinari, Juan Finello, Macarena Plaza Rojas, Franco Liberatore, Germán Robert, Santiago Otaiza-González, Pilar Velez, Martin Theumer, Patricia Agudelo-Romero, Alejandro Enet, Claudio Gonzalez, Hernan Ramiro Lascano, Laura Lucia Saavedra. Frontiers in Plant Science (2022), 13:1052358. doi: 10.3389/fpls.2022.1052358.
• “The innards of the cell: studies of water dipolar relaxation using the ACDAN fluorescent probe”. Santiago Otaiza, Manuel Cabadas, Germán Robert, Roberto P. Stock, Ramiro Lascano, Leonel Malacrida and Luis A. Bagatolli. Methods and Applications in Fluorescence (2022), 10 044010 https://doi.org/10.1088/2050-6120/ac8d4c.
• “A proposed role for endomembrane trafficking processes in regulating tonoplast content and vacuole dynamics under ammonium stress conditions in Arabidopsis root cells” Germán Robert*, Mako Yagyu, Hernán Ramiro Lascano, Céline Masclaux-Daubresse, Kohki Yoshimoto. Plant Signaling and Behavior (2021), 16(9).
• “Ammonium stress increases micro-autophagic activity while impairing macro-autophagic flux in Arabidopsis roots” Robert Germán*, Yagyu Mako, Koizumi Takaya, Naya Loreto, Masclaux-Daubresse Céline, Yoshimoto Kohki*. The Plant Journal (2020), doi: 10.1111/TPJ.15091
• “Redox systemic signaling and induced tolerance responses during soybean-Bradyrhizobium japonicum interaction: involvement of nod factor receptor and autoregulation of nodulation”. Tadeo F. Fernandez-Göbel, Rocío Deanna, Nacira B. Muñoz, Germán Robert, Sebastian Asurmendi and Ramiro Lascano. Frontiers in Plant Science (2019), 10(141) doi: 10.3389/fpls.2019.00141.
• “Phosphatidylinositol 3-kinase functions at the very early host responses during soybean- B. japonicum interaction: a local nodulation control under stress conditions?” Robert Germán, Muñoz Nacira, Alvarado-Affantranger Xochitl, Davidenco Vanina, Saavedra Laura, Rodriguez-Kessler Margarita, Estrada-Navarrete Georgina, Sánchez Federico, Lascano Ramiro. Journal of Experimental Botany (2018), 69 (8), 2037–2048.
• “Expression of animal anti-apoptotic gene Ced-9 enhances tolerance during Glycine max L.–Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation”. Germán Robert, Nacira Muñoz, Mariana Melchiorre, Federico Sánchez, Ramiro Lascano. Plos One (2014), 9 (7), e101747.
• “Negative short-term salt effects in the soybean-B. japonicum interaction and its partial reversion by the addition of calcium”. Nacira Muñoz, Marianela Rodriguez, German Robert y Ramiro Lascano. Functional Plant Biology (2014), 41, 96-105.
• “Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.-Bradyrhizobium japonicum interaction”. Nacira Muñoz; Robert German; Melchiorre Mariana; Racca Roberto; Ramiro Lascano. Environmental and Experimental Botany (2012), 78, 76 – 83.
• “Apoplastic superoxide generation and antioxidant system activity in wheat protoplast under photooxidative stress” Germán Robert, Mariana Melchiorre, Roberto Racca y Ramiro Lascano. Plant Science (2009), 177 (3): 168 – 174.
• «Superoxide Dismutase and Glutathione Reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox state» Mariana Melchiorre; Germán Robert; Victorio Trippi; Roberto Racca; Ramiro Lascano. Plant Growth Regulation (2009), 57 (1): 57 – 68.

Actividad docente

Profesor Asistente de la Cátedra de Fisiología Vegetal (docencia en las materias Fisiología vegetal; Biotecnología; Biología del desarrollo vegetal de la FCEFyN de la UNC)